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Bacteria-on-a-bead: probing the hydrodynamic interplay of dynamic cell appendages during cell separation

Nora Sauter, Matteo Sangermani, Isabelle Hug, Urs Jenal, and Thomas Pfohl. Biology Communications 5 (2022) 1093

Surface attachment of bacteria is the first step of biofilm formation and is often mediated and coordinated by the extracellular appendages, flagellum and pili. The model organism Caulobacter crescentus undergoes an asymmetric division cycle, giving rise to a motile “swarmer cell” and a sessile “stalked cell”, which is attached to the surface. In the highly polarized predivisional cell, pili and flagellum, which are assembled at the pole opposite the stalk, are both activated before and during the process of cell separation. We explored the interplay of flagellum and active pili by growing predivisional cells on colloidal beads, creating a bacteriaon-a-bead system. Using this set-up, we were able to simultaneously visualize the bacterial motility and analyze the dynamics of the flagellum and pili during cell separation. (mehr...)


In situ dissolution and swelling of confined lamellar polymer crystals through exposure to humid air

Brahim Bessif, Thomas Pfohl, Barbara Heck, Yaser Alshetwi, Emna Khechine, Jun Xu, and Günter Reiter. Macromolecules 55, 6015–6022, 2022

2022-brahim.jpgInduced by exposure to humid air, we observed in-situ the temporal and spatial changes in thickness and morphology of sandwich-like polymer platelets of a block copolymer. These micron-size platelets consisted of a crystalline poly(ethylene oxide) (PEO) lamella confined between two glassy poly(styrene) (PS) layers, formed via crystallization of a hydrophobic-hydrophilic PS-b-PEO diblock copolymer. We observed first the dissolution of the confined crystalline PEO layers characterized by sharp dissolution front propagating at a constant velocity as described by case II diffusion of a liquid into a solid. (mehr...)


Determining Entanglement Molar Mass of Glassy Polyphenylenes Using Mechanochromic Molecular Springs

Maximilian Raisch, Günter Reiter, Michael Sommer. ACS Macro Lett. 2022, 11, 760–765

2022-sommer.jpgMolecular force transduction in tough and glassy poly(meta,meta,para-phenylene) (PmmpP) was investigated as a function of Mn using covalently incorporated mechanochromic donor–acceptor torsional springs based on an ortho-substituted diphenyldiketopyrrolopyrrole (oDPP). Blending oDPP-PmmpP probe chains with long PmmpP matrix chains allowed us to investigate molar-mass-dependent mechanochromic properties for a series of specimens having mechanically identical properties.(mehr...)




Liste der Veröffentlichungen

Small-angle x-ray scattering experiments for investigating the validity of the two-phase model
G.R. Strobl, N. Müller. Journal of Polymer Science B - Polymer Physics 11, 1219-1233 (1973)
Determination of Lamellar Structure of Partially Crystalline Polymers by Direct Analysis of their Small-Angle X-Ray-Scattering Curves
G.R. Strobl. Journal of Applied Crystallography 6, 365-370 (1973)
Synthese und Eigenschaften molekulareinheitlicher n-Paraffine bis zum C140H282
W. Heitz, E.W. Fischer, R. Peters, T. Wirth, G. Strobl. Makromolekulare Chemie 162, 63 (1972)
Analyse von spaltverschmierten Kleinwinkelstreukurven lamellarer Strukturen
G.R. Strobl. Kolloid-Zeitschrift and Zeitschrift für Polymere 250, 1039-1046 (1972)
A New Method for Evaluating Slit-Smeared Small Angle X-Ray Scattering Data
G.R. Strobl. Acta Crystallographica A 26, 367-375 (1970)
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